海洋之神590手机版

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Wenwu GUO
2017-09-04

     

郭文武    博士、教授、博士生导师

男,1970年9月生,博士、教授、博士生导师。1993年7月本科毕业于海洋之神590手机版园艺系;1993-1998年,海洋之神590手机版攻读硕士、博士学位;1998年6月毕业,获博士学位并留校任教。1999、2003年,分别晋升为副教授、教授。2001-2003年,美国佛罗里达大学柑橘研究与教育中心博士后;2007年,澳大利亚CSIRO园艺部、澳大利亚国立大学高访3个月;2009年,日本鹿儿岛大学高访2个月。

通讯地址:湖北省武汉市海洋之神590手机版园艺植物生物学教育部重点实验室 430070

Tel: 027-87281543 (O);

Email: guoww@mail.hzau.edu.cn


人才团队计划、科研奖励和荣誉称号:

入选国家杰出青年科学基金(2012-2015)、国家特支计划科技创新领军人才、科技部中青年科技创新领军人才、农业部全国农业科研杰出人才(2016-2020);牵头“园艺植物细胞工程与种质创新”教育部创新团队和湖北省创新群体、“柑橘细胞工程与遗传改良”农业部创新团队;曾获中国青年科技奖、国家科技进步二等奖(第3完成人,2006),“柑橘细胞工程育种技术创新与新品种培育”获湖北省技术发明一等奖(第1完成人,2019)。


国内外学术兼职:

国际SCI刊物《Scientia Horticulturae》共同主编,《Plant Cell Tissue Organ Culture》、《Tree Genetics Genomes》Associate Editor,《Journal of Integrative Agriculture 》、《中国农业科学》、《果树学报》编委。国务院学位委员会第7届学科评议组(园艺学)成员;中国园艺学会分子育种分会副理事长。


主持承担的科研项目:

国家自然科学基金(杰青、重点、重点国际合作等)、科技部973计划课题、863计划课题、教育部项目(创新团队、重大项目培育计划、新世纪优秀人才、霍英东青年教师基金、博士点基金及优先课题)、农业部公益性行业科研专项、湖北省自然科学基金(杰青、重点、创新群体)、瑞典国际科学基金等30余项。


研究兴趣与重点内容:

从事柑橘细胞工程与遗传改良研究。针对我国柑橘在品种、品质等方面存在的问题,以及常规育种中遇到的珠心胚(无性胚)干扰等生殖障碍,借助现代分子标记和组学手段,重点开展细胞工程与遗传改良研究;旨在提高育种效率、缩短育种周期,培育具有无核、优质、不同熟期等性状的新品种,充分发挥“细胞工程”在柑橘遗传改良中的独特作用。

重点研究内容:1)细胞工程创新种质与新品种培育:体细胞胚发生机理,体细胞杂种 / 胞质杂种创制,离体培养与三倍体创制,同源多倍体发掘,纯系创制,细胞工程新品种培育;2)细胞工程新种质优异性状解析与分子育种:胞质杂种雄性不育性状解析与基因发掘,人工合成多倍体的体细胞互作遗传、表观遗传与广适应性评价,基于原生质体再生的基因编辑技术与分子育种。


发表论著:  

在Plant Phyisology、Plant Biotechnology J、J Exp Bot、J Proteome Res、DNA Res、Metabolomics、BMC Genomics、BMC Plant Biology、Theor Appl Genet、Planta、Plant Science、Plant Cell Reports、Tree Genetics Genomes、Protoplasma、Plant Cell Tissue Organ Culture、Euphytica、Scientia Horticulturae、中国农业科学、园艺学报等学术刊物发表第一和通讯作者论文100余篇。


部分论著和成果如下(* 通讯作者)

1. Wang R, Fang YN, Wu XM*, Qing M, Li CC, Xie KD, Deng XX, Guo WW* (2020) miR399-CsUBC24 module regulates the reproductive development and male fertility in citrus. Plant Physiology, accepted.

2. Tan FQ, Zhang M, Xie KD, Fan YJ, Song X, Wang R, Wu XM, Zhang HY, Guo WW* (2019) Polyploidy remodels fruit metabolism by modifying carbon source utilization and metabolic flux in Ponkan mandarin. Plant Science 289:110276.

3. Long JM, Liu CY, Feng MQ, Liu Y, Wu XM*, Guo WW* (2018) miR156-SPL modules regulate induction of somatic embryogenesis in citrus callus. Journal of Experimental Botany 69: 2979-2993.

4. Tan FQ, Tu H, Wang R, Wu XM, Xie KD, Chen JJ, Zhang HY, Xu J, Guo WW* (2017) Metabolic adaptation following genome doubling in citrus doubled diploids revealed by non-targeted metabolomics. Metabolomics 13: 143.

5. Long JM, Liu Z, Wu XM*, Fang YN, Jia HH, Xie ZZ, Deng XX, Guo WW* (2016) Genome-scale mRNA and small RNA transcriptomic insights into initiation of citrus apomixis. Journal of Experimental Botany 67: 5743-5756.

6. Fang YN, Zheng BB, Wang L, Yang W, Wu XM, Xu Q, Guo WW* (2016) High-throughput sequencing and degradome analysis reveal altered expression of miRNAs and their targets in a male-sterile cybrid pummelo (Citrus grandis). BMC Genomics 17:591.

7. Guo WW*, Liang WJ, Xie KD, Xia QM, Fu J, Guo DY, Xie ZZ, Wu XM, Xu Q, Yi HL, Deng XX (2016) Exploitation of polyploids from 39 citrus seedling populations. Acta Horticulturae 1135: 11-16.

8. Guo WW*, Xie KD, Zheng BB, Wang SM, Fang YN, Xu Q, Deng XX (2016) Molecular marker assisted cell engineering and citrus improvement. Acta Horticulturae 1127: 265-269.

9. Tan FQ, Tu H, Liang WJ, Long JM, Wu XM, Zhang HY, Guo WW* (2015) Comparative metabolic and transcriptional analysis of a doubled diploid and its diploid citrus rootstock (C. junos cv. Ziyang xiangcheng) suggests its potential value for stress resistance improvement. BMC Plant Biology 15: 89.

10. Wang SM, Lan H, Cao HB, Xu Q, Chen CL, Deng XX, Guo WW* (2015) Recovery and characterization of homozygous lines from two sweet orange cultivars via anther culture. Plant Cell Tissue Organ Culture 123:633-644.

11. Wu XM, Kou SJ, Liu YL, Fang YN, Xu Q, Guo WW* (2015) Genome-wide analysis of small RNAs in non-embryogenic and embryogenic tissues of citrus: microRNA and siRNA mediated transcript cleavage involved in somatic embryogenesis. Plant Biotechnology Journal 13: 383-394.

12. Xie KD, Xia QM, Wang XP, Liang WJ, Wu XM, Grosser JW, Guo WW* (2015) Cytogenetic and SSR marker evidence of mixed disomic, tetrasomic and intermediate inheritance in a citrus allotetraploid somatic hybrid between ‘Nova’ tangelo and ‘HB’ pummelo. Tree Genetics Genomes 11: 112.

13. Fang YN, Qiu WM, Wang Y, Wu XM, Xu Q, Guo WW* (2014) Identification of differentially expressed microRNAs from a male sterile Ponkan mandarin (Citrus reticulata Blanco) and its fertile wild type by small RNA and degradome sequencing. Tree Genetics Genomes 10: 1567-1581.

14. Xiao SX, Biswas MK, Li MY, Deng XX, Xu Q, Guo WW* (2014) Production and molecular characterization of diploid and tetraploid somatic cybrid plants between male sterile Satsuma mandarin and seedy sweet orange cultivars. Plant Cell Tissue Organ Culture 116: 81-88.

15. Xie KD, Wang XP, Biswas MK, Liang WJ, Xu Q, Grosser JW, Guo WW* (2014) 2n megagametophyte formed via SDR contributes to tetraploidization in polyembryonic ‘Nadorcott’ tangor crossed by citrus allotetraploids. Plant Cell Reports 33:1641-1650.

16. Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW* (2014) iTRAQ based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. Journal of Proteome Research 13: 2998-3015.

17. Ahmad T, Sablok G, Tatarinova T, Xu Q, Deng XX, Guo WW* (2013) Evaluation of codon biology in Citrus and Poncirus trifoliata based on genomic features and frame corrected expressed sequence tags. DNA Research 20: 135-150.

18. Guo WW*, Xiao SX, Deng XX (2013) Somatic cybrid production via protoplast fusion for citrus improvement. Scientia Horticulturae 163: 20-26.

19. Ge XX, Chai LJ, Liu Z, Wu XM, Deng XX, Guo WW* (2012) Transcriptional profiling of genes involved in embryogenic, non-embryogenic calluses and somatic embryogenesis of Valencia sweet orange by SSH based microarray. Planta 236: 1107-1124.

20. Kou SJ, Wu XM, Liu Z, Liu YL, Xu Q, Guo WW* (2012) Selection and validation of suitable reference genes for miRNA expression normalization by quantitative RT-PCR in citrus somatic embryogenic and adult tissues. Plant Cell Reports 31: 2151-2163.

21. Qiu WM, Zhu AD, Wang Y, Ge XX, Chai LJ, Deng XX, Guo WW* (2012) Comparative transcript profiling of gene expression between seedless Ponkan mandarin and its seedy wild type during floral organ development by suppression subtractive hybridization and cDNA microarray. BMC Genomics 13: 397.

22. Xu SX, Cai XD, Tan B, Li DL, Guo WW* (2011) Effect of ploidy increase on transgene expression: example from Citrus diploid cybrid and allotetraploid somatic hybrid expressing the EGFP gene. Protoplasma 248: 531-540.

23. Wu XM, Liu MY, Ge XX, Xu Q, Guo WW* (2011) Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange. Planta 233: 495-505.

24. Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW* (2009) Highly efficient transformation of GFP and KNU genes into precocious trifoliate orange (Poncirus trifoliata [L.] Raf), a potential model genotype for functional genomics studies in Citrus. Tree Genetics Genomes 5: 529-537.

25. Cai XD, Fu J, Deng XX, Guo WW* (2007) Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma and two seedy Citrus cultivars. Plant Cell Tissue Organ Culture 90: 275-283.

26. Guo WW*, Grosser JW* (2005) Somatic hybrid vigor in Citrus: direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Science 168: 1541-1545.

27. Guo WW, Duan YX, Olivares-Fuster O, Wu ZC, Arias CR, Burns JK, Grosser JW (2005) Protoplast transformation and regeneration of transgenic Valencia sweet orange plants containing a juice quality related pectin methylesterase gene. Plant Cell Reports 24: 482-486.

28. Guo WW, Prasad D, Cheng YJ, Serrano P, Deng XX, Grosser JW (2004) Targeted cybridization in Citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Reports 22: 752-758.

29. Guo WW, Cheng YJ, Deng XX (2002) Regeneration and molecular characterization of intergeneric somatic hybrids between Citrus reticulata and Poncirus trifoliata. Plant Cell Reports 20: 829-834.

30. Guo WW, Deng XX (1999) Intertribal hexaploid hybrid plants regeneration from electrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium. Theor Appl Genet 98: 581-585.

31. Guo WW, Deng XX (1998) Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Reports 18: 297-300.

32. 宋鑫,谭丰全,张苗,蔡元康,郭大勇,解凯东,伍小萌,郭文武*(2019)‘纽荷尔’脐橙与‘尤力克’柠檬种间体细胞杂种的代谢特征分析。园艺学报 46:37-46

33. 夏强明,彭珺,解凯东,伍小萌,徐强,郭文武*(2019)以雄性不育胞质杂种‘华柚2 号’为母本创制柚有性群体。果树学报 36: 961-967

34. 郭文武,叶俊丽,邓秀新*(2019)新中国果树科学研究70年—柑橘。果树学报 36: 1264-1272

35. 梁武军,解凯东,郭大勇,谢宗周,徐强,伊华林,郭文武*(2014)柑橘10个品种实生后代多倍体的发掘与SSR鉴定。园艺学报 41:409-416

36. 解凯东,王晓培,王惠芹,梁武军,谢宗周,郭大勇,伊华林,邓秀新,Grosser JW,郭文武*(2014)以柑橘多胚性二倍体母本倍性杂交培育三倍体。园艺学报 41:613-620

37. 解凯东,王惠芹,王晓培,梁武军,谢宗周,伊华林,邓秀新,Grosser JW,郭文武*(2013)单胚性二倍体为母本与异源四倍体杂交大规模创制柑橘三倍体。中国农业科学 46:4550-4557

38. 农业部植物新品种权(2015)。品种权名:华柚2号;品种权号:CNA20101034.2;品种权人:海洋之神590手机版;培育人:郭文武、程运江、谢宗周、伊华林、邓秀新

39. 授权国家发明专利(2016)。专利名称:一种早期筛选柑橘胞质杂种的方法;申请号:201310221676.9;发明人:郭文武、肖诗鑫

40. 湖北省技术发明一等奖(2019)。成果名称:柑橘细胞工程育种技术创新与新品种培育;完成人:郭文武、邓秀新、宋文化、伊华林、谢宗周、解凯东


信息于2020年6月更新

海洋之神590手机版-海洋之神发现海洋590